L-Isoleucine is an essential amino acid (which means that body cannot produce it on its own). It is the fourth most abundant amino acid in human skeletal muscle. Natural sources of L-Isoleucine include nuts, almonds, seeds, meat, cashews, eggs, brown rice, whole wheat, fish, chicken, liver, lentils, peas and soy proteins. L-Isoleucine is used in powdered form as a supplement by performance athletes and bodybuilders because it is shown to significantly boost energy. Research also shows that L-Isoleucine supplementation can boost endurance and aid in muscle recovery and repair after workouts. Deficiency of L-Isoleucine can cause headaches, dizziness, fatigue and depression.

L-Leucine, is an alpha amino acid which is extremely essential in the everyday functioning of the body. Leucine is also an essential amino acid, meaning that the human body cannot synthesize it and it has to be ingested. And is found in brown rice, nuts and whole wheat bread products. L-Leucine as an essential amino acid increases the synthesis of muscle protein and thus slows the degradation of muscle tissue. L-Leucine is also utilised in the liver, adipose tissue and muscle tissue. Simply put, L-Leucine supplies the body with energy when engaging in an athletic activity, it preserves the glucose stored in muscle tissue and it works to heal bone, skin and muscle tissue. It is also a ‘limiting nutrient’ thus it helps the body make use of the proteins ingested. Leucine deficiency can lead to depression, muscle weakness, low energy etc.

L-Valine is the third branched chain amino acid and is required for muscle metabolism, repairs and regenerates tissue, and maintains the body's nitrogen level. It also is used as a muscle energy source and preserves the use of glucose.